Backgrounds: Justicia gendarussa Burm.f. Has been known to have anti-HIV activity. This study was conducted to evaluate the effect of incubation time on the antiviral activity of the J.

Farmakope Indonesia V merupakan farmakope terakhir yang dimiliki Indonesia. Farmakope Indonesia V atau yang sering disingkat menjadi FI V merupakan acuan pemeriksaan berbagai obat di Indonesia selain farmakope lain seperti USP, Farmakope Eropa (european pharmacopeia) dan Japan Pharmacopeia. Farmakope ini berisi lebih dari 1000 monografi obat.

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Gendarussa leaves extract on HIV-infected MT-4 cells in vitro. Molecular docking test was also conducted to determine the interaction of alkaloids and flavonoids on the J. Gendarussa leaves against HIV-1 reverse transcriptase receptor. It is expected that this research will provide scientific information on the development of J. Gendarussa leaves as an anti-HIV drug. Materials and Methods: In the activity test, the effect of incubation time on the antiviral activity of J.

Gendarussa leaves on HIV-infected MT-4 cells were evaluated. During the activity test, a parameter of cytolysis effect inhibition on MT-4 cell line was observed after 4 days and 6 days incubation period. The molecular docking test is performed by using Molegro Virtual Docker software to determine the interaction of alkaloid and flavonoid compounds of J. Gendarussa leaves with HIV-1 reverse transcriptase receptor.

Introduction Human Immunodeficiency Virus (HIV) is a retrovirus that infects the human immune system. HIV damages the human immune system by destroying T lymphocyte cell (a type of leukocyte). On the T lymphocyte cell surface, there is cluster of differentiation 4(CD4) receptor. When a number of T-CD4 lymphocyte cells are low then a person more susceptible to be infected (Pattman et al., 2005). The immune system will break down slowly over the increasing number of viruses (viral load) in the body (Naif, 2013).

The final stage of HIV infection is AIDS, a condition when CD4 level decrease to very low level (typically. Plant Materials and Extraction Procedure Leaves of J. Gendarussa were obtained from a cultivated crop in Pacet, Mojokerto, East Java province, Indonesia. This plant was identified by the Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Airlangga University under the voucher number 18/H3.1.5/DT/2012. Simplicia powder of J.

Gendarussa leaves was divided into two groups: acidified leaves powder to release alkaloids and non-acidified leaves powder. Both the powder was extracted using 70% ethanol for 3x24 hours in a macerator device, and then the resulting filtrate were concentrated using a rotary evaporator.

Under these conditions, the source voltage will see a total impedance of Ztot = 1.5+j1.5 kΩ whose magnitude is 1.5√ 2 kΩ. Descargar solucionario de mecanica de materiales fitzgerald pdf gratis. The magnetization curve for neodymium-iron-boron can be represented as Bm = µRHm +Br where Br = 0.94 T and µR = 1.04µ0. Part (a): From the solution to Problem 1-46, the maximum energy product for neodymium- iron-boron at 180 C occurs at (approximately) Bm = 0.47 T and Hm = -360 kA/m. The magnetic circuit must satisfy Hmd+Hgg = 0; BmAm = BgAg For Bg = 0.8 T, the minimum magnet volume will occur when the magnet is operating at the maximum energy point.

The extract is dried at 50°C a temperature of to obtain a 70% ethanol extract (17.4% w/w) and fractionated-70% ethanol extract (6.4% w/w) of J. Gendarussa leaves. Water extract of leaves of J.

Gendarussa is made by blending fresh J. Gendarussa leaves in cold water. Then the resulted filtrate was collected and was dried using the freeze-dry method to obtain water extracts (1.8% w/w) of J. Gendarussa leaves. Alkaloid and Flavonoid Screening of J. Gendarussa Leaves Extract Alkaloid screening of 70% ethanol extract, fractionated-70% ethanol extract and water extract of J. Gendarussa leaves be performed using a TLC GF254 plate with mobile phase chloroform: methanol (9:1).